Virucidal Short Wavelength Ultraviolet Light Treatment of Plasma and Factor VI 11 Concentrate : Protection of Proteins by Antioxidants

نویسندگان

  • Ehud Ben-Hur
  • John Hamman
  • Rongyu Jin
  • Edward Dubovi
  • Bernard Horowitz
چکیده

The use of solvent/detergent mixtures and various forms of heat treatment to inactivate viruses has become widespread in the preparation of blood derivatives. Because viruses that lack lipid envelopes and/or are heat resistant, eg, hepatitis A virus (HAV) or parvovirus B19 may be present, the use of two methods of virus elimination that operate by different mechanisms has been advocated. We now report on short wavelength ultraviolet light (UVC) irradiation for virus inactivation and enhancement of its compatibility with proteins by quenchers of reactive oxygen species (ROS). Treatment of an antihemophilic factor (AHF) concentrate or whole plasma with 0.1 J/cm2 inactivated 10' t o 2 10' infectious doses (ID) of encephalomyocarditis virus (EMCV), HAV, bacteriophage M13, vesicular stomatitis virus (VSV), and porcine parvovirus. However, the recovery of factor Vlll was 30% or lower

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تاریخ انتشار 2000